Pineapple protease is a group of sulfhydryl hydrolytic proteases extracted and refined by bioengineering technology from pineapple, with a molecular weight of 33,000 and an isoelectric point of 9.55. Pineapple protease is mainly derived from the plant stem, so it is also called stem pineapple protease. The main component of pineapple protease is a protease containing sulfhydryl group, which also contains peroxidase, acid phosphate esterase, several protein inhibitors and organic active calcium, whose active center is sulfhydryl group (-SH), which can carry out various protein hydrolysis and biochemical reactions, and can be widely used in food, pharmaceutical and biological industries.
Pineapple protease or pineapple extract is a compound composed of proteases usually found in pineapple. While it is widely known for its ability to aid in the digestion and breakdown of proteins, it actually does much more than that. The good news is that pineapple protease is commercially available as a supplement and as an aid in the treatment of disease.
The history of bromelain is inextricably linked to pineapples, as they are the only known major source of this group of enzymes. Pineapple proteases were first discovered by chemist Vicente Marcano in 1891 and subsequently extracted by another chemist, Russell Henry Chittenden. The English name for bromelain was originally "bromelin", described as "the protein hydrolase in pineapple juice". The first major source was the pineapple fruit, as scientists found that ripe pineapple stems had higher levels of bromelain.
After its discovery, the production of pineapple protease has expanded over the years. Its commercialization led manufacturers to develop faster extraction techniques for mass production and purification. Today, bromelain supplements are used for numerous health uses.
It has been observed that the effect of bromelain depends on the time of day it is taken. For example, if taken with a meal, it can help promote digestion and absorption, but if taken on an empty stomach, it can help relieve inflammation.
The only major source of pineapple protease is the pineapple plant. This enzyme is usually extracted from pineapple stems or young pineapple fruit, but other parts of the pineapple plant have been found to have lower levels of bromelain. No other fruit has been found to contain significant amounts of bromelain. Depending on the location of extraction, pineapple protease mixtures are currently divided into two types. They are.
1. stem bromelain (SBM) - This type of bromelain is the most common on the market because it is cheaper to extract and the pineapple stem is essentially of no other value. Its extraction is carried out by centrifugation, ultrafiltration or lyophilization. When pineapple protease is mentioned or used in mainstream products, it is most likely referring to this type.
2. fruit bromelain (FBM) - According to studies, fruit bromelain has lower digestive activity than stem bromelain, but its protein hydrolytic activity is better. Unlike stem pineapple protease, this type of pineapple protease is not widely utilized due to its limited availability.
1. Pineapple protease can act on the aging keratin layer on human skin, prompting its degradation, decomposition and removal, promoting skin metabolism, reducing the phenomenon of dark skin color caused by sun exposure, so that skin maintenance presents a good white state.
2. pineapple protease is a natural, non-toxic, non-side effect protein, as a sulfhydryl hydrolytic protease, can carry out the hydrolysis of proteins, peptide chain of protein molecules hydrolysis to generate peptides or amino acids, the formation of protein hydrolysate with a unique flavor.
3. Bromelain has been proven to be a safe and effective therapeutic drug in the field of medicine where it prevents platelet agglutination, fibrinolysis, anti-inflammatory effects, cytokine and immunomodulatory effects, skin debridement, facilitates the absorption of other drugs, mucolytic effects, aids digestion, promotes wound healing, and improves the cardiovascular and circulatory systems. Bromelain is well absorbed orally and data show that it is more effective in high doses.
COA Of Bromelain Price
| Product Name | Bromelain | Extraction Solvent | Ethanol&Water |
| Source | Ananas comosus (Linn.) Merr. | Plant Part | Stem |
| ITEM | SPECIFICATION | TEST METHOD |
| Physical Description | ||
| Appearance | Light Yellow Powder | Visual |
| Odor | Characteristic | Organoleptic |
| Taste | Characteristic | Olfactory |
| Chemical Tests | ||
| Enzyme Activity | ≥1200GDU/g | GDU method |
| Loss on drying | ≤10g/100g | GB5009.3 |
| Ash | ≤6g/100g | GB5009.4 |
| Cadmium (Cd) | ≤1 ppm | CP2015(AAS) |
| Mercury (Hg) | ≤1 ppm | CP2015(AAS) |
| Lead (Pb) | ≤2 ppm | CP2015(AAS) |
| Arsenic (As) | ≤2ppm | CP2015(AAS) |
| Microbiology Control | ||
| Aerobic bacterial count | ≤10,000 cfu/g | CP2015 |
| Total Yeast & Mold | ≤100 cfu/g | CP2015 |
| Escherichia coli | Negative | CP2015 |
| Salmonella | Negative | CP2015 |
| Staphlococcus Aureus | Negative | CP2015 |
The optimum reaction temperature is 30-45℃, pH value is 6-6.8, and the addition amount is 0.2-1.0%. The optimum temperature and pH value change according to the type and concentration of the substrate, and the reaction time should be shortened or extended accordingly.
The optimum amount of enzyme must be added according to the specific production situation, please write or call for more information.
1. Pineapple protease comes from natural plants, with the characteristics of safety, non-toxic, wide range of action.
2. Using advanced biological engineering technology: made by microfiltration, ultrafiltration, membrane separation and vacuum freeze-drying, the product quality reaches food and pharmaceutical grade standards, and has passed the national export food production enterprises health standards certification.
1. Application in food processing industry.
Baked food; for casein coagulation; bromelain hydrolyzes large molecules of meat protein into easily absorbed small molecules of amino acids and proteins, which can be widely used in the finishing of meat products.
2. In medicine, health care products industry applications
Bromelain can inhibit the growth of tumor cells; as a protein hydrolase is beneficial to the prevention and treatment of cardiovascular disease; bromelain can selectively remove the skin, so that the new skin transplantation can be carried out as early as possible; bromelain will be used in combination with various antibiotics to improve their efficacy; it can promote the transmission of antibiotics at the site of infection, thereby reducing the amount of antibiotics used. In addition, pineapple protease can promote the absorption of nutrients.
3. Application in beauty and cosmetic industry
Pineapple protease has excellent efficacy of skin rejuvenation, whitening and spot removal